World's only instant tutoring platform
Search questions and Textbooks
dropdown-logo
Instant TutoringAbout Us
Login
App StorePlay Store
Get 2 FREE Instant-Explanations on Filo with code FILOAPP
CBSE
Arrow Icon
Which of the following tools of recombinant DNA technology is
Question

Question asked by Filo student

rotate Arrowrotate Arrow
Which of the following tools of recombinant DNA technology is incorrec

Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

  1. EcORI - Production of sticky ends
  2. DNA ligase - Multiplication of rDNA molecules
  3. ori-copy number
  4. Selectable marker-Identification of transformants
Views

Views: 5,523 students

Updated on: Mar 12, 2023

tutor 0tutor 1tutor 2
Found 4 tutors discussing this question
Michael Discussed
Which of the following tools of recombinant DNA technology is incorrectly paired with its use?
5 mins ago
Discuss this question LIVE
5 mins ago
Video Solution

Filo tutor solutions (1)

Learn from their 1-to-1 discussion with Filo tutors.

filo Logo
2 mins

Uploaded on: 3/12/2023

Ask your question, on a video call with tutor

Connect instantly with this tutor

Connect now

Taught by

tutor profile picture
Om Prakash Bishnoi

Total classes on Filo by this tutor - 1,038

Teaches : Biology, Physics, Mathematics

Connect instantly with this tutor

Connect now

Notes from this class (3 pages)

Arrow
Download
Arrow
Arrow
Arrow
Was this solution helpful?
100
Share
Report
One destination for complete JEE/NEET preparation
One destination to cover all your homework and assignment needs
Learn Practice Revision Succeed
Instant 1:1 help, 24x7
Instant 1:1 help, 24x7
60, 000+ Expert tutors
60, 000+ Expert tutors
Textbook solutions
Textbook solutions
Big idea maths, McGraw-Hill Education etc
Big idea maths, McGraw-Hill Education etc
Essay review
Essay review
Get expert feedback on your essay
Get expert feedback on your essay
Schedule classes
Schedule classes
High dosage tutoring from Dedicated 3 experts
High dosage tutoring from Dedicated 3 experts
Trusted by 4 million+ students

Practice more questions on Biotechnology

Question 1
Medium
Views

Views: 5,286

Disadvantages of chemical agents are :
I. chemicals are toxic and harmful to human beings and animals
II. chemical pollute the environment and plants
III. weedicides used to remove weeds also pollute the soil
Choose the correct option :
  1. I, II and III
  2. I and II
  3. I and III
  4. II and III
Topic

Topic:

Microbes in Human Welfare

View solution

Question 2
Easy
Views

Views: 5,916

Name the interaction in each of the following:
(a) growing on a shoe flower plant.
(b) Mycorrhizae living on the roots of higher plants.
(c) Clown fish living among the tentacles of sea anemone.
(d) Koel laying its eggs in crow's nest.
Topic

Topic:

Organisms and Populations

Book

Book:

Trueman's Biology for NEET (Truemans)

View solution

Question 3
Easy
Views

Views: 5,934

The amount of usable energy, which is available for doing work, when the temperature and pressure are uniform throughout the system is called
  1. Enthalpy
  2. Activation energy
  3. Free energy
  4. Spontaneous energy
Topic

Topic:

Ecosystem

View solution

Question 4
Easy
Views

Views: 6,169

What did the Miller-Urey experiment prove?
  1. That life arose on the Earth through abiogenesis.
  2. That life could have arisen on the Earth through biogenesis.
  3. That life on the Earth did not arise through abiogenesis.
  4. It proved none of the above.
Topic

Topic:

Evolution

View solution

View more

Students who ask this question also asked

Question 1
Views

Views: 5,216

dirouf ol pores incubating the cells with recombinant DNA to Ting nes ence such as isolation of DNA, fragmentation of DNA by cells and how on of the DNA fragment into a vector, transferring the. example. (on minant DNA into the host, culturing the host cells in a ats is able, mbinant DNA into the host, culturing the host cells in a 1 animals the (us examine each of these steps in somerial (DNA) 3 cells. A ogens in ogens in all that nucleic acid is the genetic material of all organisms these interest ant aut exception. In majority of organisms theic or DNA. In order to cut the DNA with cterium sriction enzymes, it needs to be in pure form, free from other cabranes, we have to break the cell open to release DNA along rith other macromolecules such as RNA, proteins, wysaccharides and also lipids. This can be achieved by treating te bacterial cells/plant or animal tissue with enzymes such as rsozyme (bacteria), cellulase (plant cells), chitinase (fungus). iou know that genes are located on long molecules of DNA aterwined with proteins such as histones. The RNA can be removed by treatment with ribonuclease whercas proteins can be removed by tratment with protease. Other molecules can be removed by appropriate tratments and pun? DNA ultimately precipitates out after the addition of chilled ethanol. This can be seen as collection of fine threads in the suspension (Figure 11.5).
Topic

Topic:

Biotechnology

View solution

Question 2
Views

Views: 5,131

gene gets 'inactivated due to insertion' of alien DNA, and helps in selection of recombinants. Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure because it requires simultaneous plating on two plates having different antibioties. Therefore, aiternative selectable markers have beenbinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, -galactosidase. This results into inactivation of the gene for synthesis of this enzyme, which is referred to as insertional inactivation. The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. Presence of insertresults into insertional inactivation of the these are identified as recombinant colonies. (iv) Vectors for cloning genes in plants and animals : You may be surprised to know that we have learnt the lesson of transferring genes into plants and animals from bacteria and viruses which have knoina this for ages - how to deliver genes to transform eukaryotic cells and force them to do what the bacteria or viruses want. For example, Agrobacterium tumifaciens, a pathogen of several dicot plants isable to deliver a piece of DNA known as 'T-DNA' to transform normal plant cells into a tumor and direct these tumor cells to produce the aus examine eacl chemicals required by the pathogen. Similarly, retroviruses in animals better understanding of the art of delivering genes by pathogensin their eukaryotic hosts has generated knowledge to transform thest dhat nucleic ac tools of pathogens into useful vectors for delivering genes of intera sout exception. to humans. The tumor inducing (Ti) plasmid of Agrobacteriur Mnbomucleic acidicis tumifaciens has now been modified into a cloning vector which is titimeng aymes, it more pathogenic to the plants but is still able to use the mechanisnit: to deliver genes of our interest into a variety of plants. Sinilarive retroviruses have also been disarmed and are now used to delint desirable genes into animal cells. So, once a gene or a DNA fragnerterl has been ligated into a suitable vector it is transferred into a bactei: plant or animal host (where it multiplies). 11.2.3 Competent Host (For Transformation with Recombinant DNA)
Topic

Topic:

Biotechnology

View solution

Question 3
Views

Views: 5,866

or Non- Bt) and why? b. Cotton Bollworms were introduced in another experimental study on the above farm lands wherein no pesticide was used. Explain what effect would a Bt and Non Bt crop have on the pest.
Topic

Topic:

Biotechnology

View solution

Question 4
Views

Views: 5,612

In order to make the host cell competent Divalent cations such as calcium are used-
  1. The divalent ions have to be in a specific concentration
  2. They cause the DNA uptake by the cell
  3. They increase the efficiency with which DNA enters the bacterium through pores in its cell wall.
  4. All of the above
Topic

Topic:

Biotechnology

View solution

View more
Doubt Icon Doubt Icon

Stuck on the question or explanation?

Connect with our Biology tutors online and get step by step solution of this question.

231 students are taking LIVE classes
Question Text
Which of the following tools of recombinant DNA technology is incorrectly paired with its use?
Updated OnMar 12, 2023
TopicBiotechnology
SubjectBiology
ClassClass 12
Answer Type Video solution: 1
Upvotes100
Avg. Video Duration2 min