World's only instant tutoring platform
dropdown-logo
Question

Question asked by Filo student

In order to make the host cell competent Divalent cations such as calcium are used-

  1. The divalent ions have to be in a specific concentration
  2. They cause the DNA uptake by the cell
  3. They increase the efficiency with which DNA enters the bacterium through pores in its cell wall.
  4. All of the above
In order to make the host cell competent Divalent cations such as calc
expand image
Video Solution

Video solutions (1)

Learn from their 1-to-1 discussion with Filo tutors.

filo Logo
6 mins

Uploaded on: 3/19/2022

Ask your question, on a video call with tutor
Was this solution helpful?
133
Share
Report
tutor 0tutor 1tutor 2
Found 4 tutors discussing this question
Discuss this question LIVE
8 mins ago
One destination for complete JEE/NEET preparation
One destination to cover all your homework and assignment needs
Learn Practice Revision Succeed
Instant 1:1 help, 24x7
Instant 1:1 help, 24x7
60, 000+ Expert tutors
60, 000+ Expert tutors
Textbook solutions
Textbook solutions
Big idea maths, McGraw-Hill Education etc
Big idea maths, McGraw-Hill Education etc
Essay review
Essay review
Get expert feedback on your essay
Get expert feedback on your essay
Schedule classes
Schedule classes
High dosage tutoring from Dedicated 3 experts
High dosage tutoring from Dedicated 3 experts
Trusted by 4 million+ students

Students who ask this question also asked

Question 1
Views

Views: 5,608

lons lor also. (angode) (9.4) Insertion of Recombinant DNA into the Host arse several methods of introducing the ligated DNA into recipient seclplent cells after making them 'competent' to receive, take up Apresenitin its surrounding. So, if a recombinant DNA bearing gene (the host cells become transformed into ampicillin-resistant . coll . med the transformed cells on agar plates containing ampicillin, If comants will grow, untransformed recipient cells will die. Since, due cpicllin resistance gene, one is able to select atransformed cell in the sance of ampicillin. The ampicillin resistance gene in this case is called wectable marker. (3,5 0btaining the Foreign Gene Product (ayou insert a plece of alien DNA into a cloning vector and transfer it a bacterial, plant or animal cell, the alien DNA gets multiplied. In witall recombinant technologies, the ultimate aim is to produce a able protein. Hence, there is a need for the recombinant DNA to be issed. The foreign gene gets expressed under appropriate conditions. cexpression of foreign genes in host cells involve understanding many incal details. After having cloned the gene of interest and having optimised the (iltions to induce the expression of the target protein, one has. to Alder producing it on a large scale. Can you think of any reason (there is a need for large-scale production? If any protein encoding Fis expressed in a heterologous host, it is called a recombinant meln. The cells harbouring cloned genes of interest may be grown a small scale in the laboratory. The cultures may be used for (recting the desired protein and then purifying it by using different vation techniques. Thecells can also be multiplied in a continuous culture system wherein
Question 2
Views

Views: 5,490

this a recombinant DNA is inserted within the codingled tare an enzype, gene for synthesis of this endyme, which is relerred to as insertichend inaetivation. The presence of a chromogenic substrate gliven these are Identified as recombinant colonies. (iv) Vectors for cloning genes in plants and animals : You mati, surprised to know that we have learnt the lesson of transieming if into plants and animals from bacteria and viruses whict have ken of force them to do what the bacteria or viruses want. For exament plant cells into a tumor and direet these tumor cells to produen chemicals required by the pathogen. Similarly, retroviruses in anime better understanding of the art of delivering genes by pathogens in fithat nuclelc to humans. The tumor inducing (Ti) plasmid of Agrabacierte to hifactens has now been modified into a cloning vector whichis more pathogenic to the plants but is still able to use the mechanisher to deliver genes of our interest into a variety of plants. Similar plant or animal host (where it muliplies). 11.2.3 Competent Host (For Transformation with Recombinant DNA) Since DNA is a hydrophilic molecule, it cannot pass through col membranes. Why? In order to force bacteria to take up the plasmid. the bacterial cells must first be made 'competent' to take up DNA. This done by treating them with a specific concentration of a divalenteation de chilled eth such as calcium, which increases the efficiency with which DNA entef fujension
View more
Doubt Icon Doubt Icon

Stuck on the question or explanation?

Connect with our Biology tutors online and get step by step solution of this question.

231 students are taking LIVE classes
Question Text
In order to make the host cell competent Divalent cations such as calcium are used-
Updated OnMar 19, 2022
TopicBiotechnology
SubjectBiology
ClassClass 12
Answer Type Video solution: 1
Upvotes133
Avg. Video Duration6 min