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UV Biology lab manual EXIERCISE - 2 : POULEN GERMINATION Principle: Artificially pollen grains are germinate in synthetic medium. During germination, the misese grows out as pollen tube through one of the germ pores in the exine. Requirements: Mature pollen grains, $10\%$ sucrose solution (10g sucrose dissolved in $100\mathrm{ml}$ of $\mathrm{Hg}$. Petridish, slides, cover slips, brush, needle and microscope etc. Procedure:

1. Take a drop of $10\%$ sucrose solution on a clean cover slip and dust the mature pollen grains onit.
2. Place the cover slip inverted on to a clean slide.
3. After 10 minutes observe the slide under microscope
4. Count total number of pollengrains seen in the microscopic field and count the number of polien grains that have germinated.

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