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The internal environment consists of ___________.a. all body fluidsb. all fluids in cellsc. all body fluids outside cellsd. interstitial fluid

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Texts: When using the blue-white screening technique, the blue colonies that form on the culture plate indicate that the bacterial cells have taken up a vector that has an intact lacZα gene, meaning there is no foreign DNA inserted into the gene. As a result, the bacteria can break down X-gal and produce a blue color. On the other hand, the white colonies do not break down X-gal and instead appear white, which indicates the presence of foreign DNA inserted into the lacZα gene. This insertion disrupts the formation of an active β-galactosidase, resulting in the absence of blue color formation. 1. Comment on the number of white colonies on your plates: are they more abundant than blue colonies? What determines the ratio of white versus blue colonies on the plate? Tube # dilution # mls type of plate: plated Number of Colonies on the plate confluent Transformants/ml Average (transformants/ml) total transformants T1 (no DNA) T1 (no DNA) None 0.1 No Antibiotic XXXXXXXX XXXXXXXX|XXXXXXX None 0.1 Amp + IPTG 0 + X-gal XXXXXXXX XXXXXXXX XXXXXXX 10^(-1) 0.1 Amp + IPTG 1412 (912 blue and 500 white) Amp + IPTG 124 (80 blue and 44 white) Amp + IPTG 3 (2 blue and 1 white) Amp + IPTG 4 (3 blue and 1 white) Amp + IPTG 12 (10 blue and 2 white) Amp + IPTG 32 (24 blue and 8 white) Amp + IPTG 6 (3 white and 3 blue) Amp + IPTG 10 (7 blue and 3 white) Amp + IPTG 1 (white) Amp + IPTG 2 (white) Amp + IPTG 16 (7 blue and 9 white) Amp + IPTG 9 (8 white and 1 blue) 132,600 8,751,600 T2 (Native) 10^(-2) 0.1 None 0.1 25 30 T3 (RC) None 0.2 None 0.1 90 108 T4 (LC) None 0.2 None 0.1 55 66 T5 (PC) None 0.2 None 0.05 41.25 49.25 None 0.1 T6 (L) None 0.2 None 0.2
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Question Text
The internal environment consists of ___________.a. all body fluidsb. all fluids in cellsc. all body fluids outside cellsd. interstitial fluid
TopicAll topics
SubjectBiology
ClassClass 11