Texts: What plasmid vector would you use to conduct the following experiment? List the components required in the vector and draw a picture of the vector. Please use illustrations, including labels, with your answer. Also, include how you would select for the vector in the host organism (not E. coli). You may refer to the PowerPoint "Genome Structure" for clues. Each question is worth 3.3 points. 1. You wish to knock out the yeast gene, RRT15, in the yeast chromosome. 2. Express your favorite human gene in yeast on a plasmid. 3. Add a carboxy-terminal YFP gene in-frame at the chromosomal locus to an existing yeast gene.

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