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Please help with this question....... (9 points) Two Patients A (solid curve) and B (dashed curve) went to a particularly well-instrumented cardiac catheterization lab and had the pressure-volume cycles of the left ventricle during heartbeat assessed. 200 180 160 140 120 100 80 60 40 20 0 Which patient (A or B) has the higher diastolic arterial pressure? (b) Which patient (A or B) has the higher afterload? Which patient (A or B) has the higher right atrial pressure?

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You would like to rapidly generate two different knockout mice using CRISPR-Cas9. The genes to be knocked out are Pcsk9 and Apoc3, both involved in lipid metabolism. In each case, you would like to take advantage of non-homologous end joining (NHEJ) to introduce frameshift mutations into the coding sequence of the gene. You begin by choosing the gene exons within which to introduce mutations. You use the UCSC Genome Browser (www.genome.ucsc.edu) to assess the exon-intron structure of each gene. You use four tracks to show each gene: (1) UCSC Genes (2) Ensembl Genes (3) RefSeq Genes (4) Other RefSeq Genes (this shows orthologs from other species). Introns are portrayed as lines with arrows showing the direction of transcription. Exons are portrayed as bars. Within the exons, the thick bars indicate coding sequence, and the thin bars indicate noncoding sequence (i.e., 5' UTR and 3' UTR). You note that in the mouse genome, both genes are transcribed from the "minus" strand (i.e., in the reverse direction). Mouse Genome Browser View for Pcsk9 gene Mouse Genome Browser View for Apoc3 gene Gene Editing Design after "looking" at the gene: You see that for at least one of the genes, there are several different annotated transcripts. This raises the possibility that putting a frameshift mutation into the "first" exon of the longest transcript may not actually knock out the gene, since it would not affect all of the transcripts. A similar situation could arise if you targeted an alternatively spliced exon. At the same time, you want to target as early as possible in the coding sequence so that a frameshift mutation will truncate/disrupt as much of the protein as possible. 1. Which exon (counting from right to left, since the gene is transcribed in the reverse direction) would you target in Pcsk9 to ensure that you knock out the gene? 2. Based on the results returned by the CRISPR server, choose four 20-bp protospacer sequences and enter them below. [If the server is taking too long to return the results, you can use the link http://bit.ly/CRISPORpage]
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Please help with this question....... (9 points) Two Patients A (solid curve) and B (dashed curve) went to a particularly well-instrumented cardiac catheterization lab and had the pressure-volume cycles of the left ventricle during heartbeat assessed. 200 180 160 140 120 100 80 60 40 20 0 Which patient (A or B) has the higher diastolic arterial pressure? (b) Which patient (A or B) has the higher afterload? Which patient (A or B) has the higher right atrial pressure?
TopicAll topics
SubjectBiology
ClassClass 11