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Measurement of intracellular calcium levels in the brain and s
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Measurement of intracellular calcium levels in the brain and spinal cord was conducted using synaptosomes prepared on a Ficoll density gradient. The synaptosomes were incubated with fura-2AM and then washed to remove excess fura-2. Basal and KCl-stimulated levels of intracellular calcium were determined using the SPEX Cation Measurement System. The maximum and minimum binding of fura-2 to calcium were determined to calculate the free intracellular calcium levels. The formula used for calculation was [Ca++]i = Kd )< (F - Fmin)/(Fmax - F) x Sf2/Sb2. (Pugh et al., 1994)

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Measurement of intracellular calcium levels in the brain and spinal cord was conducted using synaptosomes prepared on a Ficoll density gradient. The synaptosomes were incubated with fura-2AM and then washed to remove excess fura-2. Basal and KCl-stimulated levels of intracellular calcium were determined using the SPEX Cation Measurement System. The maximum and minimum binding of fura-2 to calcium were determined to calculate the free intracellular calcium levels. The formula used for calculation was [Ca++]i = Kd )< (F - Fmin)/(Fmax - F) x Sf2/Sb2. (Pugh et al., 1994)
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Question Text
Measurement of intracellular calcium levels in the brain and spinal cord was conducted using synaptosomes prepared on a Ficoll density gradient. The synaptosomes were incubated with fura-2AM and then washed to remove excess fura-2. Basal and KCl-stimulated levels of intracellular calcium were determined using the SPEX Cation Measurement System. The maximum and minimum binding of fura-2 to calcium were determined to calculate the free intracellular calcium levels. The formula used for calculation was [Ca++]i = Kd )< (F - Fmin)/(Fmax - F) x Sf2/Sb2. (Pugh et al., 1994)
TopicAll topics
SubjectBiology
ClassClass 11