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Let's suppose you performed the steps of gene cloning described in Figures 21.2 and Which experiment(s) would you conduct to confirm that a white colony really contained a recombinant vector withan insert?a. Pick a white bacterial colony and restreak it on plates containing X-Gal to confirm that the cells really form white colonies.b. Pick a white bacterial colony, isolate plasmid DNA, digest the plasmid DNA with a restriction enzyme, and then perform gel electrophoresis with the DNA.c. Pick a white bacterial colony and test it to see if -galactosidase is functional within the bacterial cells.d. Pick a white bacterial colony and retest it on ampicillin-containing plates to double-check that the cells are really ampicillin resistant.e. Both and should be conducted.
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Question Text | Let's suppose you performed the steps of gene cloning described in Figures 21.2 and Which experiment(s) would you conduct to confirm that a white colony really contained a recombinant vector withan insert?a. Pick a white bacterial colony and restreak it on plates containing X-Gal to confirm that the cells really form white colonies.b. Pick a white bacterial colony, isolate plasmid DNA, digest the plasmid DNA with a restriction enzyme, and then perform gel electrophoresis with the DNA.c. Pick a white bacterial colony and test it to see if -galactosidase is functional within the bacterial cells.d. Pick a white bacterial colony and retest it on ampicillin-containing plates to double-check that the cells are really ampicillin resistant.e. Both and should be conducted. |
Topic | All topics |
Subject | Biology |
Class | Class 12 |