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Which of the following has popularised the PCR (polymerase chain reactions)?
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During in -vitro polymerisation of DNA, the reaction undergoes cycles of heating and cooling. The parent DNA is denatured at C. Then it is cooled to about so that DNA primers can attach themselves to the DNA template. The extension of the primer takes place at in presence of enzyme Taq polymerase which is a DNA polymerase that remains stable at this high temperature.
So, the correct answer is 'Availability or Thermostable DNA polymerase'
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| Question Text | Which of the following has popularised the PCR (polymerase chain reactions)? |
| Answer Type | Text solution:1 |
| Upvotes | 150 |
