What does PCR stand for? Describe the different steps of PCR.

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Filo · Accepted Answer

PCR stands for polymerase chain reaction. It is a process which is used to create copies of DNA fragment. In this, PCR reaction mixture contains Taq polymerase, primers, template DNA and nucleotides There are three steps of PCR namely, denaturation step, annealing step and extension step. 1. Denaturation step: In this step, the reaction is  heated at 940C in order to denature the DNA strands. This results in a single-stranded template.2. Annealing step: In this step, the reaction is cooled down to 550C to 650C in order to bind primer to their complementary sequences on the single-stranded template DNA.3. Extension: This is the last step of PCR process. In this step, the temperature of reaction is raised to 720C so that Taq polymerase enzyme synthesizes new strands of DNA by extending primer.This cycle repeats 25 to 35 times in the entire process.

Question Text	What does PCR stand for? Describe the different steps of PCR.
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What does PCR stand for? Describe the different steps of PCR.

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