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The heat shock method in bacterial transformation is to facili
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The heat shock method in bacterial transformation is to facilitate :

A
Binding of DNA to the cell wall

B
Uptake of DNA through membrane transport proteins

C
Uptake of DNA through transient pores in the bacterial cell wall

D
Expression of antibiotic resistance gene

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Updated on: Nov 27, 2022

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The heat shock method is done at 42°C, pores formed in the cell membrane become larger and DNA enters the cells. Heat shock method in presence of a bivalent cation like creates transient pores in the cell wall allowing the uptake of foreign DNA present in the medium.
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Question Text
The heat shock method in bacterial transformation is to facilitate :
Updated OnNov 27, 2022
TopicBiotechnology: Principles and Processes
SubjectBiology
ClassClass 12
Answer TypeText solution:1 Video solution: 1
Upvotes153
Avg. Video Duration20 min