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Read the passage and answer the following question:
In order to prevent changes in volume due to osmosis, unfertilized frog oocyte was bathed in Frog Ringer's solution. The Frog Ringer's solution was supplemented with radiolabeled amino acids. A sample of oocytes was taken every 30 minutes and assayed for radioactivity. At time 1, the oocytes were fertilized. Samples were taken at five-minute intervals after fertilization and assayed for radioactivity. The results are presented in the graph.

How could a control be created for this experiment?

A
Immerse the oocytes in non-radiolabeled Frog Ringer's solution prior to fertilization.

B
Sample the oocytes at five-minute intervals prior to fertilization.

C
Sample the oocytes at 30-minute intervals after fertilization.

D
Divide the oocytes into two groups, immerse one group in non-radiolabeled Frog Ringer's solution and one group in radiolabeled solution.

E
Divide the oocytes into two groups, fertilize one group, and simulate fertilization in the other, but do not actually introduce sperm into the oocytes.

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Question Text
Read the passage and answer the following question:
In order to prevent changes in volume due to osmosis, unfertilized frog oocyte was bathed in Frog Ringer's solution. The Frog Ringer's solution was supplemented with radiolabeled amino acids. A sample of oocytes was taken every 30 minutes and assayed for radioactivity. At time 1, the oocytes were fertilized. Samples were taken at five-minute intervals after fertilization and assayed for radioactivity. The results are presented in the graph.

How could a control be created for this experiment?