Reaction of 1989 was

Nicking by restriction endonucleases

Polymerase chain reaction

Reverse genetics

Splicing

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In 1985 Norm Arnheim, also a member of the development team concluded his sabbatical at Cetus and assumed an academic position at USC. He began to investigate the use of PCR to amplify samples containing just a single copy of the target sequence. By 1989 his lab developed multiplex-PCR on single sperm to directly analyze the products of meiotic recombination.

So, the correct answer is 'Polymerase chain reaction.'

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