Describe the process of amplification of ''gene of interest '' using PCR technique.

Question

Filo · Accepted Answer

Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. The process involves the following steps: Denaturation: The double-stranded DNA is heated up to  94oC which causes the hydrogen bonds to break and the two strands get separated.Annealing: The two sets of primers are added which bind to the appropriate complementary segment of the DNA strand at 54oC.Extension: The Taq polymerase enzyme polymerizes the nucleotide chain using the nucleotides provided in the medium and by using the template strand at 72oC.

Question Text	Describe the process of amplification of ''gene of interest '' using PCR technique.
Answer Type	Text solution:1
Upvotes	150

Describe the process of amplification of ''gene of interest '' using PCR technique.

Text solutionVerified

Practice questions from similar books