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A mixture of fragmented DNA was electrophoresed in an agarose
Biology Exemplar
Biology Exemplar

Class 12

NCERT Exemplar

Publisher:
NCERT
Subject:
Biology
1

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2

Chapter 2: Sexual Reproduction in Flowering Plants

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3

Chapter 3: Human Reproduction

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4

Chapter 4: Reproductive Health

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5

Chapter 5: Principles of Inheritance and Variation

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6

Chapter 6: Molecular Basis of Inheritance

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7

Chapter 7: Evolution

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8

Chapter 8: Human Health and Disease

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9

Chapter 9: Strategies for Enhancement in Food Production

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10

Chapter 10: Microbes in Human Welfare

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11

Chapter 11: Biotechnology: Principles and Processes

48 questions

Q1
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA b and, while linear DNA shows two fragments. Explain.
Q2
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
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12

Chapter 12: Biotechnology and its Applications

52 questions

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13

Chapter 13: Organisms and Populations

70 questions

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14

Chapter 14: Ecosystem

68 questions

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15

Chapter 15: Biodiversity and Conservation

61 questions

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16

Chapter 16: Environmental Issues

52 questions

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Question
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Solving time: 3 mins

A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?

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Updated on: Jan 22, 2023

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Biology Exemplar (NCERT Exemplar)

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A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
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The reasons are as follows
(i)  DNA sample that was loaded on the gel may have got contaminated with nuclease (exo or endo both) and completely degraded.
(ii)  Electrodes were put in opposite orientation in the gel assembly that is anode towards the wells (where DNA sample is loaded). Since, DNA molecules are negatively charged, they move towards anode and hence, move out of the gel instead of moving into the matrix of gel.
(iii)   Ethidium bromide was not added at all or was not added in sufficient concentration and so DNA was not visible.
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Question Text
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
Updated OnJan 22, 2023
TopicBiotechnology: Principles and Processes
SubjectBiology
ClassClass 12
Answer TypeText solution:1 Video solution: 1
Upvotes221
Avg. Video Duration2 min